This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0022580; Intron-spanning
This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich furin-like domains and one thrombospondin type 1 domain. In mice the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing providing a protective effect against chemotherapy-induced adverse effects. [provided by RefSeq Jul 2008]