PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MRPL35, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCID0022318; Intron-spanning

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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5024372
Chromosome Location:   2:86434402-86437688question
Amplicon Length:   105

Gene Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species the proteins comprising the mitoribosome differ greatly in sequence and sometimes in biochemical properties which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p 10q and Xp. [provided by RefSeq Jul 2008]

Gene Symbol:   MRPL35
Gene Name:   mitochondrial ribosomal protein L35
Aliases:   L35mt, MRP-L35
RefSeq:   NC_000002.11 NG_013037.1 NT_022184.15
Ensembl:   ENSG00000132313
Entrez:   51318
UniGene:   Hs.433439
Chromosome Mapping:   2p11.2

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