This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0022264; Intron-spanning
Molybdenum cofactor biosynthesis is a conserved pathway leading to the biological activation of molybdenum. The protein encoded by this gene is involved in this pathway. This gene was originally thought to produce a bicistronic mRNA with the potential to produce two proteins (MOCS1A and MOCS1B) from adjacent open reading frames. However only the first open reading frame (MOCS1A) has been found to encode a protein from the putative bicistronic mRNA whereas additional splice variants whose full-length natures have yet to be determined are likely to produce a fusion between the two open reading frames. This gene is defective in patients with molybdenum cofactor deficiency type A. A related pseudogene has been identified on chromosome 16. [provided by RefSeq Jan 2010]