This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0021578; Intron-spanning
Dihydrouridine synthase catalyzes reduction of the 56-double bond of a uridine residue on the displacement loop of tRNA. The resultant modified base 56-dihydrouridine appears to increase the conformational flexibility and dynamic motion of tRNA (Kato et al. 2005 [PubMed 15994936]).[supplied by OMIM Mar 2008]