This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0020833; Intron-spanning
This gene encodes several androgen-dependent epididymis-specific secretory proteins. The specific functions of these proteins have not been determined but they are thought to be involved in sperm maturation. Some of the isoforms contain regions of similarity to beta-defensins a family of antimicrobial peptides. The gene is located on chromosome 8p23 near the defensin gene cluster. Alternative splicing of this gene results in seven transcript variants encoding different isoforms. Two different N-terminal and five different C-terminal protein sequences are encoded by the splice variants. Two additional variants have been described but their full length sequences have not been determined. [provided by RefSeq Jul 2008]