This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0020314; Intron-spanning
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq Jun 2011]