This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0020225; Intron-spanning
This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 1 a related family member on chromosome 3. Heterozygous deletion of these linked genes is involved in Dandy-Walker malformation which is a congenital cerebellar malformation. Multiple transcript variants have been identified for this gene. [provided by RefSeq Dec 2009]