This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0018408; Intron-spanning
Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown cell cycle regulation and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains which is mediated by ubiquitin-specific proteases (USPs) such as USP25 (Valero et al. 1999 [PubMed 10644437]).[supplied by OMIM Mar 2008]