This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0018283; Intron-spanning
The protein encoded by this gene is involved in both the synthesis and degradation of fructose-26-bisphosphate a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-26-bisphosphate and a fructose-26-biphosphatase activity that catalyzes the degradation of fructose-26-bisphosphate. This protein regulates fructose-26-bisphosphate levels in the heart while a related enzyme encoded by a different gene regulates fructose-26-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Jul 2008]