This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0018091; Intron-spanning
This gene is one of three myosin V heavy-chain genes belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1) Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported but the full-length nature of some variants has not been determined. [provided by RefSeq Dec 2008]