This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0018000; Intron-spanning
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis smooth muscle contraction the formation of actin stress fibers and focal adhesions and the activation of the c-fos serum response element. This protein which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq Jul 2008]