This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0017538; Intron-spanning
This gene encodes a protein that is secreted by white adipocytes and which plays a major role in the regulation of body weight. This protein which acts through the leptin receptor functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions and is involved in the regulation of immune and inflammatory responses hematopoiesis angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq Jul 2008]