PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SPAM1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCID0017341; Intron-spanning

List Price:    $134.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5022440
Chromosome Location:   7:123589933-123593492question
Amplicon Length:   149

Gene Information

Hyaluronidase degrades hyaluronic acid a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte a receptor that plays a role in hyaluronic acid induced cell signaling and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2010]

Gene Symbol:   SPAM1
Gene Name:   sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
Aliases:   HYA1, HYAL1, HYAL3, HYAL5, MGC26532, PH-20, PH20, SPAG15
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000106304
Entrez:   6677
UniGene:   Hs.121494
Chromosome Mapping:   7q31.3

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