PrimePCR™ ddPCR™ Expression EvaGreen® Assay: RPE65, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCID0017033; Intron-spanning

List Price:    $134.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5022185
Chromosome Location:   1:68912471-68914356question
Amplicon Length:   93

Gene Information

This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein a soluble form called sRPE65 and a palmitoylated membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT) the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa. [provided by RefSeq Jul 2008]

Gene Symbol:   RPE65
Gene Name:   retinal pigment epithelium-specific protein 65kDa
Aliases:   LCA2, RP20, mRPE65, rd12, sRPE65
RefSeq:   NC_000001.10 NG_008472.1 NT_032977.9
Ensembl:   ENSG00000116745
Entrez:   6121
UniGene:   Hs.2133
Chromosome Mapping:   1p31

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