This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0016934; Intron-spanning
The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein which is a member of the peptidase C1 protein family is predominantly expressed in osteoclasts. However the encoded protein is also expressed in a significant fraction of human breast cancers where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing. [provided by RefSeq Jul 2008]