This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0016666; Intron-spanning
The protein encoded by this gene has weak homology to several carbohydrate kinases a class of proteins involved in the phosphorylation of sugars as they enter a cell inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences frequently involved in chromosomal rearrangements. [provided by RefSeq Jul 2008]