This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0016426; Intron-spanning
This gene encodes a member of the vanin family of proteins which share extensive sequence similarity with each other and also with biotinidase. The family includes secreted and membrane-associated proteins a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins however they possess pantetheinase activity which may play a role in oxidative-stress response. This protein like its mouse homolog is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to and in the same transcriptional orientation as two other vanin genes on chromosome 6q23-q24. [provided by RefSeq Feb 2009]