This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0016009; Intron-spanning
The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1384-amino acid protein also designated p190 or CASPR for 'contactin-associated protein' includes an extracellular domain with several putative protein-protein interaction domains a putative transmembrane domain and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins and this protein may be the signaling subunit of contactin enabling recruitment and activation of intracellular signaling pathways in neurons. [provided by RefSeq Jan 2009]