This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0015518; Intron-spanning
This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle increasing more than 10-fold during the latter part of G1. [provided by RefSeq Jul 2008]