This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0015410; Intron-spanning
The mineralized portions of teeth the dentin and enamel are formed by mesenchyme-derived odontoblasts and epithelium-derived ameloblasts respectively. As ameloblasts differentiate they deposit specific proteins necessary for enamel formation including amelogenin (AMELX; MIM 300391) enamelin (ENAM; MIM 606585) and ameloblastin (AMBN; MIM 601259) in the organic enamel matrix. Amelotin is specifically expressed in maturation-stage ameloblasts (Iwasaki et al. 2005 [PubMed 16304441]).[supplied by OMIM Mar 2008]