This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0014986; Intron-spanning
This gene encodes the pro-alpha1 chains of type III collagen a fibrillar collagen that is found in extensible connective tissues such as skin lung uterus intestine and the vascular system frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV and with aortic and arterial aneurysms. Two transcripts resulting from the use of alternate polyadenylation signals have been identified for this gene. [provided by R. Dalgleish Feb 2008]