This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0014888; Intron-spanning
This gene encodes a protein which contains multiple domains including a CXXC zinc finger three PHD zinc fingers two FY-rich domains and a SET (suppressor of variegation enhancer of zeste and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene however the full length nature of the shorter transcript is not known. [provided by RefSeq Jul 2008]