PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CHEK2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCID0014511; Intron-spanning

List Price:    $134.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5020115
Chromosome Location:   22:29107897-29115459question
Amplicon Length:   156

Gene Information

In response to DNA damage and replication blocks cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated the encoded protein is known to inhibit CDC25C phosphatase preventing entry into mitosis and has been shown to stabilize the tumor suppressor protein p53 leading to cell cycle arrest in G1. In addition this protein interacts with and phosphorylates BRCA1 allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also mutations in this gene are thought to confer a predisposition to sarcomas breast cancer and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Apr 2012]

Gene Symbol:   CHEK2
Gene Name:   CHK2 checkpoint homolog (S. pombe)
Aliases:   CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53
RefSeq:   NC_000022.10 NG_008150.1 NT_011520.12
Ensembl:   ENSG00000183765
Entrez:   11200
UniGene:   Hs.291363
Chromosome Mapping:   22q11,22q12.1

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