PrimePCR™ ddPCR™ Expression EvaGreen® Assay: WT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCID0014203; Intron-spanning

List Price:    $134.00
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Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5019901
Chromosome Location:   11:32417814-32421550question
Amplicon Length:   167

Gene Information

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern with biallelic and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq Oct 2010]

Gene Symbol:   WT1
Gene Name:   Wilms tumor 1
Aliases:   AWT1, GUD, NPHS4, WAGR, WIT-2, WT33
RefSeq:   NC_000011.9 NT_009237.18 NG_009272.1
Ensembl:   ENSG00000184937
Entrez:   7490
UniGene:   Hs.591980
Chromosome Mapping:   11p13

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