This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0014174; Intron-spanning
Crystallins are separated into two classes: taxon-specific or enzyme and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development these crystallins are made and then retained throughout life making them extremely stable proteins. Mammalian lens crystallins are divided into alpha beta and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs a connecting peptide and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E gamma-F gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes gamma-crystallins have been involved in cataract formation. [provided by RefSeq Jul 2008]