PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HLA-DRB5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

ddPCR Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.

Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013959; Intron-spanning

List Price:    $134.00
Your Price:   Log In
 

Assay Information

Technology:   ddPCR
Assay Type:   EvaGreen
Application:   Gene Expression
Unique Assay ID:   dHsaEG5019719
Chromosome Location:   6:32489869-32498039question
Amplicon Length:   191

Gene Information

HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide exons 2 and 3 encode the two extracellular domains exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3 DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1 otherwise it is omitted. There are 4 related pseudogenes: DRB2 DRB6 DRB7 DRB8 and DRB9. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-DRB5
Gene Name:   major histocompatibility complex, class II, DR beta 5
Aliases:   FLJ76359, HLA-DRB
RefSeq:   NC_000006.11 NG_002432.1 NT_007592.15
Ensembl:   ENSG00000198502
Entrez:   3127
UniGene:   Not Available
Chromosome Mapping:   6p21.3

Number Description Download
10033487 ddPCR™ Mutation Detection Assays Product Insert, Ver C Click to download
10033173 ddPCR™ Copy Number Variation Assays Product Insert, Ver C Click to download
10041702 PrimePCR™ ddPCR™ Gene Expression Assays (EvaGreen) Product Insert, Rev A Click to download
10047489 ddPCR™ Mutation Detection Assays Product Insert, Ver B Click to download
10048730 PrimePCR™ ddPCR™ Gene Expression Probe Assays Product Insert, Rev A Click to download