This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0013887; Intron-spanning
Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events including cell signaling proliferation cytoskeletal organization and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs) guanine nucleotide-releasing factors (GRFs) and GDP-dissociation inhibitors (GDIs). The GDIs including ARHGDIB decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al. 1993 [PubMed 8356058]).[supplied by OMIM Dec 2010]