This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0013873; Intron-spanning
This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides orexin A and orexin B by proteolytic processing. Orexin A and orexin B which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2 function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior metabolism and homeostasis. [provided by RefSeq Jan 2010]