This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0013705; Intron-spanning
The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal a 500-amino acid sema domain and 16 conserved cysteine residues (Kolodkin et al. 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes all of which also have an immunoglobulin domain. The semaphorin III family consisting of human semaphorin III (SEMA3A; MIM 603961) chicken collapsin and mouse semaphorins A D and E all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al. 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al. 1995 [PubMed 7637782]) a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM Mar 2008]