This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0013645; Intron-spanning
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13 which may contribute to many overlapping functions of this cytokine and IL13. STAT6 a signal transducer and activator of transcription has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene IL3 IL5 IL13 and CSF2 form a cytokine gene cluster on chromosome 5q with this gene particularly close to IL13. This gene IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq Jul 2008]