This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0013324; Intron-spanning
Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis namely the conversion of D-fructose 6-phosphate to D-fructose 16-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits PFK from liver is a homotetramer of L-subunits while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq Aug 2008]