This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0012807; Intron-spanning
This gene encodes transthyretin one of the three prealbumins including alpha-1-antitrypsin transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition affecting predominantly peripheral nerve and/or the heart and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy euthyroid hyperthyroxinaemia amyloidotic vitreous opacities cardiomyopathy oculoleptomeningeal amyloidosis meningocerebrovascular amyloidosis carpal tunnel syndrome etc. [provided by RefSeq Jan 2009]