This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0012672; Intron-spanning
Cholesterol homeostasis is regulated at least in part by sterol regulatory element (SRE)-binding proteins (e.g. SREBP1; MIM 184756) and by liver X receptors (e.g. LXRA; MIM 602423). Upon sterol depletion LXRs are inactive and SREBPs are cleaved after which they bind promoter SREs and activate genes involved in cholesterol biosynthesis and uptake. Sterol transport is mediated by vesicles or by soluble protein carriers such as steroidogenic acute regulatory protein (STAR; MIM 600617). STAR is homologous to a family of proteins containing a 200- to 210-amino acid STAR-related lipid transfer (START) domain including STARD5 (Soccio et al. 2002 [PubMed 12011452]).[supplied by OMIM Mar 2008]