This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0012516; Intron-spanning
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis although those complexes at the kinetochore remain. Therefore the encoded protein is thought to be an important part of functional kinetochores. In addition this protein interacts with BRCA1 and is phosphorylated by ATM indicating a potential role for this protein in DNA repair. This gene which belongs to the SMC gene family is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq Jul 2008]