This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0012509; Intron-spanning
This gene encodes a cyclin-dependent kinase inhibitor which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes and thus controls the cell cycle progression at G1. The degradation of this protein which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq Jul 2008]