This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0012258; Intron-spanning
This gene encodes a protein that plays a role in desmosome assembly cell adhesion cytoskeletal organization and epidermal differentiation. This protein co-localizes with desmoplakin and the cytolinker protein periplakin. In general this protein localizes to the nucleus desmosomes cell membrane and cortical actin-based structures. Some isoforms of this protein also associate with microtubules. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity has not been verified. [provided by RefSeq Aug 2011]