This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0012228; Intron-spanning
Accumulation of neurotoxic amyloid-beta is a major hallmark of Alzheimer disease (AD; MIM 104300). Formation of amyloid-beta is catalyzed by gamma-secretase (see PSEN1; MIM 104311) a protease with numerous substrates. PION or GSAP selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and its substrate the amyloid-beta precursor protein (APP; MIM 104760) C-terminal fragment (APP-CTF) (He et al. 2010 [PubMed 20811458]).[supplied by OMIM Nov 2010]