This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0011683; Intron-spanning
The human genome includes many retroelements including the human endogenous retroviruses (HERVs). ERV3 one of the most studied HERVs is thought to have integrated 30 to 40 million years ago and is present in higher primates with the exception of gorillas. Taken together the observation of genome conservation the detection of transcript expression and the presence of conserved ORFs is circumstantial evidence for a functional role. A functional role is also suggested by the observation that downregulation of ERV3 is reported in choriocarcinoma. [provided by RefSeq Jul 2008]