This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0011636; Intron-spanning
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells such as those in testis thymus fetal liver and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and in vitro to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene therefore may regulate cell proliferation. This protein also phosphorylates histone casein and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq Jul 2008]