This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0011007; Intron-spanning
This gene encodes a protein that is one of more than 20 subunits of the mediator complex first identified in S. cerevisiae that is required for activation of transcription. The product of this gene also interacts with elongins B and C and CUL2 and RBX1 to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq Jul 2008]