This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0010874; Intron-spanning
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs) and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD a TNF receptor associated apoptotic signal transducer ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1 an apoptosis inhibitor possessing ubiquitin ligase activity can unbiquitinate and induce the degradation of this protein and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene but the biological validity of only one transcript has been determined. [provided by RefSeq Jul 2008]