This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0010786; Intron-spanning
DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this DEAD box protein family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21 also called RH-II/GuA have similar genomic structures and are in tandem orientation on chromosome 10 suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2 3 and 4. Alternative splicing of this gene generates multiple transcript variants but the full length nature of all the other variants but one has not been defined. [provided by RefSeq Jul 2008]