This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0010562; Intron-spanning
The protein encoded by this gene contains SAM ARF-GAP RHO-GAP ankyrin repeat RAS-associating and pleckstrin homology (PH) domains. In vitro this protein displays RHO-GAP and phosphatidylinositol (345) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2008]