This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0010403; Intron-spanning
This gene encodes a member of the plakin protein family of adhesion junction plaque proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the full-length nature of some variants has not been defined. It has been reported that some isoforms are expressed in neural and muscle tissue anchoring neural intermediate filaments to the actin cytoskeleton and some isoforms are expressed in epithelial tissue anchoring keratin-containing intermediate filaments to hemidesmosomes. Consistent with the expression mice defective for this gene show skin blistering and neurodegeneration. [provided by RefSeq Mar 2010]