This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0010173; Intron-spanning
This gene is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain an actin-binding domain a neck domain that interacts with other proteins and a tail domain that serves as an anchor. This gene encodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness reduced vestibular function and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jul 2008]