This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0010131; Intron-spanning
This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli catenin beta-1 glycogen synthase kinase 3 beta protein phosphate 2 and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein alone and in a complex with other proteins has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma hepatoblastomas ovarian endometriod adenocarcinomas and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Dec 2010]