This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0010120; Intron-spanning
This gene encodes a member of the hook family of coiled-coil proteins which bind to microtubules and organelles through their N- and C-terminal domains respectively. The encoded protein localizes to discrete punctuate subcellular structures and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified but the full-length nature of some of these variants has not been determined. [provided by RefSeq Jul 2008]