This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0009876; Intron-spanning
NEPH1 is a member of the nephrin-like protein family which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766) and the genes are expressed in kidney podocytes cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al. 2003 [PubMed 12424224]).[supplied by OMIM Mar 2008]