This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0006688; Intron-spanning
The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs both in normal mRNA turnover and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq Jun 2011]