This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ Evagreen assay for gene expression analysis. EvaGreen assays consist of unlabeled PCR primer.
Info: EG; Same primer pair as used in qPCR assay qHsaCID0006481; Intron-spanning
This gene encodes the sixth largest subunit of RNA polymerase II the polymerase responsible for synthesizing messenger RNA in eukaryotes that is also shared by the other two DNA-directed RNA polymerases. In yeast this polymerase subunit in combination with at least two other subunits forms a structure that stabilizes the transcribing polymerase on the DNA template. [provided by RefSeq Jul 2008]